| Title |
Detection of Kanamycin - Resistant Bacteria and Genes in Miho and Sukanm Creeks by Southern Hybridization |
| Authors |
서보승 ; 김치경 ( Bo Seung Seo ; Chi Kyung Kim ) |
| Abstract |
The kanamycin-resistant bacteria and genes were detected by Southern hybridization with Km gene probe from the less polluted Miho creek and Suknam creek heavily polluted with sewage and industrial waste. The average values of BOD, phosphorus, conductivity, and turbidity of Suknam creek water were much higher than those of Miho creek water. Standard plate count and Km bacterial count of Suknam creek water were 6.7 × 10^5 and 5.2 × 10⁴ CFU/㎖, respectively. Those counts were about 70 to 85 times higher than those of Miho creek water, but the ratios of Km` bacterial count to standard plate count in both creeks were almost the same to be 6.2∼7.8 × 10^(-2). The bacterial strains hybridized with Km` gene probe were detected to be 39.7% in Suknam creek and 31.8% in Miho creek. The highest ratio of the hybridization was observed in Autumn season in both creek waters. When the Km` gene was localized by DNA hybridization in the Km` strains, about 40% of them isolated from Suknam creek was found to have the Km` gene in the chromosomal DNA and 59% in both chromosomal DNA and plasmid. But in the Km` strains isolated from Miho creek 37% was observed to have the Km` gene in chromosomal DNA and 53% in both chromosomal DNA and plasmid. The strains which had the Km` gene in both chromosomal DNA and plasmid were observed at highest ratio in Autumn and Winter in Suknam creek, but only in Winter in Miho creek. |