| Title |
Cadmium Accumulation in the Cell of Cadmium - Tolerant Bacteria , Pseudomonas putida , and Recovery of Cadmium from the Cells Accumulating Cadmium |
| Authors |
조주식 ; 임영성 ; 허종수 ( Ju Sik Cho ; Yeong Sung Lim ; Jong Soo Heo ) |
| Abstract |
Cadmium-tolerant bacteria, Pseudomonas putida which possessed the ability to accumulate cadmium, was isolated from mine wastewater polluted with various heavy metals. The characteristics of cadmium accumulation in the cells, recovery of the cadmium from the cells accumulating cadmium, were investigated. Removal rate of cadmium from the solution containing 100㎎/ℓof cadmium by Pseudomonas putida was more than 87% at 48 hours after inoculation of the cells. A large number of the electron-dense granules were found mainly on the cell wall and cell membrane fractions, when determined by transmission electron microscope. Energy dispersive X-ray spectroscopy revealed that the electron-dense granules were cadmium complex with the substances binding cadmium. The cadmium accumulated into the cells was not desorbed by distilled water, but more than 80% of the cadmium accumulated was desorbed by 0.1M-EDTA solution. The residues of the cells after combustion at 550% amounted to about 21% of the dry weight of the cells. EDS analysis showed that the residues were comparatively pure cadmium compound containing more than 81% of cadmium. |