| Title |
Analysis of Microcystins in Daecheong Reservoir using by High Performance Liquid Chromatography |
| Authors |
유순주(Soon Ju Yu),천세억(Se Uk Cheon),윤영삼(Young Sam Yoon),황종연(Jong Yeon Hwang),한의정(Eui Jung Han),유재근(Jae Keun Ryu) |
| Abstract |
Bloom-forming cyanobacteria are commonly observed in eutrophic water bodies and pose a serious water quality problem because of the potent toxins that they often produce. The most commonly reported toxins are the hepatotoxins the largest group being heptapeptides, known as microcystins. It is known that microcystins are produced within the cyanobacterial cell and become free in the water column after cell lysis. It is therefore important not only to develop a rapid method of analysis but also to provide a mean of assessing intracellunar levels of toxins in addition to those free in water. The filtered water the cell were subjected to trace enrichment using a C_(18) solid-phase extraction disk and cartridge, followed by identification and determination by photodiode-array high performance liquid chromatography. Recoveries of microcystin-RR, YR, LR in ultra pure water were found to be good when spiked with concentrations as low as 1㎍/l. The linearity and precision of the experimental procedure were assessed for these microcystins. The procedures were assessed using water and the cell sampled in Daecheong reservoir where has been observed water blooming in summer seasons. |