| Title |
Estrogenic Activity Assay of Endocrine Disruptors by Fluorescence Polarization System |
| Authors |
김시준(Shi Jun Kim),김정숙(Jeong Sook Kim) |
| Abstract |
Endocrine disruptors(EDs) consist of synthetic and naturally occurring chemicals that affect the balance of normal hormonal functions in animals. Depending on their activity that they may be characterized as estrogen modulators or androgen modulators. Estrogenic activity of endocrine disruptors was assayed with fluorescence polarization system. 17β-estradiol, nonylphenol, bisphenol A and benzophenone that estrogenic activity is suspected were used for the competition assay. Because, there is much detection frequency in a municipal wastewater as compared with other endocrine disruptors in Japan. Fluorescence polarization in high concentration of 5,000nM of the endocrine disrupters which were 17β-estradiol, nonylphenol, bisphenol A and benzophenone were 100.8mP, 162.6mP. 288.6mP and 330.2mP respectively. The IC_(50)(50% inhibition concentration) of 17β-estradiol, nonylphenol, bisphenol A and benzophenone were 25nM. 3,000nM, 5 × l0⁴nM and 2 × l0^6nM respectively. As a results. It was estimated that strength of estrogenic activity of the endocrine disrupters was the order of 17β-estradiol, nonylphenol, bisphenol A and benzophenone. |