| Title |
Comparison Chromogenic Enzyme Substrate Test with the Standard Multiple Tube Fermentation Method and Membrane Filtration Method for Detection of Coliforms in Drinking Water |
| Authors |
장현정 ( Hyun Jeong Jang ) ; 이목영 ( Mok Young Lee ) ; 최선영 ( Sun Young Choi ) ; 이의광 ( Eu Kwang Lee ) ; 오세종 ( Sea Jong Oh ) ; 박수환 ( Soo Whan Park ) |
| Abstract |
The chromogenic enzyme substrate test(MMO-MUG) is a method based on technology that uses a hydrolyzable substrate as a specific indicator nutrient for the target microbes. This method is a simultaneously enumerate E. coli and total coliforms(TC) in drinking water without confirmatory test. Over a 6 months period(`99. 9.∼11. and `00. 6.∼8.), 2005 samples from drinking water distribution system were analyzed for TC, and E. coli by chromogenic enzyme substrate test(MMO-MUG), multiple tube fermentation(MTF) and membrane filtration(MF) method, using replicate 100mL sample volumes. The detection rate of TC by MMO-MUG, MTF and MF method were 1.4%, 1.4% and 1.1% respectively, The agreement ratio of MMO-MUG to MF and MMO-MUG to MTF were 99.3%(1991/2005) and 99.0%(1985/2005), respectively. The Overall agreement ratio was 98.6%. The comparison of the agreement ratios by nonparametric statistics demonstrated no significant difference. Species identifications from positive samples confirmed the sensitivity of the MMO-MUG(96%) So the MMO-MUG, MTF and MF used in this study, while not showing differences available in terms of overall consequence, made a few differences in terms of quickness, easiness and costs. |