| Title |
Evaluation of Riverine Microbial Diversity using the Culture-Independent Genetic Fingerprinting Technique (T-RFLP) |
| Authors |
정주용 ( Ju Yong Jeong ) ; 이경희 ( Kyong Hee Lee ) |
| Keywords |
Culture-dependent; Ecological indexes; Riverine; Terminal restriction fragment Length polymorphism; T-RFLP |
| Abstract |
To analyze the riverine microbial community structure, genetic fingerprints and ecological indexes such as species abundances, diversity, evenness, dominance of targeted rivers in Gyeonggi Province were acquired and evaluated using terminal restriction fragment length polymorphism (T-RFLP) technique. Genetic fingerprinting technique such as T-RFLP, which is able to show the microbial community clearly unlike traditional culture-dependent techniques, was thought to be useful to analyse the riverine microbial ecosystem under various factors. Riverine ecosystem evaluation using visible organisms would give biased results with time, targeted organism and researcher. But, T-RFLP, which can exclude the subjected biases such as culture condition and identification, would be an option to understand natural ecosystem by including the microorganisms that defy culture but perform important functions. |