| Title |
Reduction of Estrogenic Activity by Gamma-ray Treatment |
| Authors |
강성욱 ( Sung Wook Kang ) ; 서재환 ( Jae Hwan Seo ) ; 이병천 ( Byoung Cheun Lee ) ; 김수진 ( Sue Jin Kim ) ; 정진호 ( Jin Ho Jung ) |
| Keywords |
Endocrine disrupting chemicals; Estrogenic activity; Radiation treatment; Yeast two-hybrid assay |
| Abstract |
In ibis study, degradation of estrone (E1) and l7α-ethynylestradiol (EE2) by gamma-irradiation and subsequent reduction of estrogenic activity as a function of absorbed dose were conducted using the yeast two-hybrid assay. Relative potency of E1 and EE2 compared to estrogenic activity of l7β-estradiol (E2) was found to be 0.0144 and 0.1605, respectively. More than 90% of E1 and EE2 (both 5.0 x 10(-6) M) was removed at an absorbed dose of 5 kGy, but more than 40% of estrogenic activity still remained. The addition of TiO2 catalyst appeared to improve the removal efficiency of E1 and decrease estrogenic activity while there was no significant effect for EE2. Additionally, the calculated estrogenic activity of E1 and EE2 based on a regression model was well correlated with the observed activity. |