| Title |
Fermentative Water Purification based on Bio-hydrogen |
| Authors |
이정열 ( Jung Yeol Lee ) ; ( Xue Jiao Chen ) ; 민경석 ( Kyung Sok Min ) |
| Keywords |
Bio-hydrogen; Clostridium butyricum; Co-culture; Dark fermentation; Photo fermentation; Rhodobacter sphaeroides |
| Abstract |
Among various techniques for hydrogen production from organic wastewater, a dark fermentation is considered to be the most feasible process due to the rapid hydrogen production rate. However, the main drawback of it is the low hydrogen production yield due to intermediate products such as organic acids. To improve the hydrogen production yield, a co-culture system of dark and photo fermentation bacteria was applied to this research. The maximum specific growth rate of R. sphaeroides was determined to be 2.93 h-1 when acetic acid was used as a carbon source. It was quite high compared to that of using a mixture of volatile fatty acids (VFAs). Acetic acid was the most attractive to the cell growth of R. sphaeroides, however, not less efficient in the hydrogen production. In the co-culture system with glucose, hydrogen could be steadily produced without any lag-phase. There were distinguishable inflection points in the accumulation of hydrogen production graph that resulted from the dynamic production of VFAs or consumption of it by the interaction between the dark and photo fermentation bacteria. Lastly, the hydrogen production rate of a repeated fed-batch run was 15.9 mL-H2/L/h, which was achievable in the sustainable hydrogen production. |