| Title |
Inactivation of Mycobacterium using Ultrasonic and Ultraviolet Sequential Processes |
| Authors |
김완기 ( Wan Gi Kim ) ; 정연정 ( Yeon Jung Jung ) ; 윤여준 ( Yeo Joon Yoon ) ; 임관훈 ( Gwan Hun Lim ) ; 김종배 ( Jong Bae Kim ) ; 강준원 ( Joon Wun Kang ) |
| Keywords |
Inactivation; M. marinum; Ultrasonic; US-UV sequential process; UV |
| Abstract |
In this study, the inactivation efficiency of Mycobacterium marinum was evaluated in buffered water (pH 7) using a low pressure ultraviolet (LP-UV) lamp, ultrasonic (US), and UV/US sequential processes. In the UV alone process, 3 log inactivation of the M. marinum was achieved with a UV dose of 120 mJ/cm2. However, a tailing phase was later observed because M. marinum has a high tendency for cell aggregation. Even though the M. marinum was not inactivated in the US alone process, the hydrophobicity decreased and turbidity increased due to the crumbling of the cell aggregation. Among the candidate processes which were UV alone, US-UV sequential process and UV-US-UV sequential process, the US-UV sequential process showed the highest synergistic effects for M. marinum inactivation. Consequently, US is a very useful process as a UV irradiation pre-treatment to inactivate M. marinum in water. |