| Title |
The Experimental Model Development of Antibiotic Resistance Gene Transfer Characteristics with Various Micropollutants |
| Authors |
김두철 ( Doo Cheol Kim ) ; 오준식 ( Jun Sik Oh ) ; 김성표 ( Sung Pyo Kim ) |
| Keywords |
Antibiotic Resistant Gene; ARG; pB10 plasmid; Escherichia coli DH5α; Pseudomonas aeruginosa |
| Abstract |
Recently, antibiotic resistant genes (ARGs) in the environment are emerging as pollutants, since these genetic contaminants can eventually be transferred to human pathogens. The aim of this study was to develop the experimental model of antibiotic resistant gene (ARG) plasmid transfer as a function of various environmental conditions. For this purpose, the multi drug resistant plasmid pB10, which is known to be originally isolated from a wastewater treatment plant, was selected as a model transfer plasmid and Escherichia coli DH5α containing pB10 was used as a model donor. Pseudomonas aeruginosa, an opportunistic pathogen, was selected as the recipient for the conjugation experiment. When the donor and recipient were exposed to various stressors including antibiotics and heavy metal as a function of the concentrations (10, 100 and, 1000 ppb), statistically increased plasmid transfer rate was observed at a concentration of 10 ppb of tetracycline and sulfamethoxazole compared to control (no antibiotic exposure). Accordingly, the developed experimental ARG model by various stressor is a promising tool for evaluating the dissemination of ARGs by micro-contaminants in aquatic environment. |