| Title |
Cloning of Heat Shock Protein 70 and Its Expression Profile under an Increase of Water Temperature in Rhynchocypris kungangensis |
| Authors |
임지수 ( Ji Su Im ) ; 길성호 ( Sung Ho Ghil ) |
| Keywords |
β-actin; global warming; heat shock protein; Rhynchocypris kumgangensis; water temperature |
| Abstract |
Water temperature is key factor influencing growth and reproduction of fish and its increase give rise to various physiological changes including gene expression. Heat shock protein(Hsp), one of the molecular chaperones, is highly conserved throughout evolution and its expression is induced by various stressors such as temperature, oxidative, physical and chemical stresses. Here, we isolated partial cDNA clones encoding 70 kDa Hsp(Hsp70) and B actin using reverse transcriptase PCR(RT PCR) from gut of Rhynchocypris kumgangensis, a Korean indigenous species and cold water fish, and investigated expression profiles of Hsp70 under an increase of water temperature using B action as an intermal control for RT PCR. Cloned Hsp70 cDNA of R. kumgangensis showed homology to Ctenopharyngodon idella (96%), Hypophthalmichthys molitrix (96%). Danio rerio (93%) and Oncorhynchus mykiss(81%) Hsp70. Cloned B actin cDNA of R. kumgangensis shouwed homology to D. rerio (98%), H, molitrix (97%), C. idella(97%) and O. mykiss(90%) B actin, Both mRNA of Hsp70 and B actin were expressed in gut, brain, and liver in R. kumgangensis. Futhermore, expression of Hsp70, in brain, was highly augmented by an increase of water temperature. These results suggest that Hsp70 mRNA expression level in brain can be used as a biological molecular marker to represent physiological stress against an increase of water temperature. |