Title |
Interactive Toxic Effects of Heavy Metals and Diesel on Vibrio fischeri |
Authors |
정현 ( Hyun Jung ) ; 박숙현 ( Soo Khyun Park ) ; 황유식 ( Yu Sik Hwang ) |
DOI |
https://doi.org/10.15681/KSWE.2014.30.4.403 |
Keywords |
Diesel; Heavy metal; Interactive effect; Lead; Vibrio fischeri; Zinc |
Abstract |
The toxicity of heavy metals (Zn, Pb) and diesel, in single and binary solution was investigated using the photobacterium Vibrio fischeri (Microtox test) as a test organism. In this experiment, the concentration of water soluble fraction of diesel was based on the total petroleum hydrocarbon (TPH). The toxicity of each single compound showed the following EC50 (15min): Zn 1.90 mg/L, Pb 0.31 mg/L, TPH 2.09 mg/L. The observed toxicity of binary mixtures increased, depending on the concentration of the mixed substance. The effects were defined as synergistic, antagonistic, or additive, in accordance with the sign of difference between the predicted and observed toxicity at binary mixtures. The interactive effects between zinc and lead were synergistic, on the other hand, antagonistic and additive effects were found in each metal and TPH mixtures on the bioluminescence of V. fischeri. |