| Title |
A Study on Comparison of Phycocyanin Extraction Methods for Hyperspectral Remote Sensing of Cyanobacteria in Turbid Inland Waters |
| Authors |
하림 ( Rim Ha ) ; 신현주 ( Hyunjoo Shin ) ; 남기범 ( Gibeom Nam ) ; 박상현 ( Sanghyun Park ) ; 강태구 ( Taegu Kang ) ; 송현오 ( Hyunoh Song ) ; 이혁 ( Hyuk Lee ) |
| DOI |
https://doi.org/10.15681/KSWE.2016.32.6.520 |
| Keywords |
Cyanobacteria; Extraction methods; Hyperspectral sensing; Phycocyanin; Pigment analyses |
| Abstract |
Phycocyanin (PC) is one of the water-soluble accessory pigments of cyanobacteria species, and its concentration is used to estimate the presence and relative abundance of cyanobacteria. In laboratory experiments PC content of field data were determined using Sarada`s freeze-thaw method in algal bloom season. The effectiveness of three selected extraction methods (repeated freeze-thaw method, homogenization, power control) for PC. It was found that the extraction efficiency of phycocyanin was the highest (of the methods compared) when a single freezing-thawing cycle was followed by pre-sonication. Applying this optimized method to surface water of Korean inland waters, the average concentration distribution was estimated 2.9~51.9 mg/m3. It has been shown that the optimized pre-sonication method is suitable to measure cyanobacteria PC content for the characterization of inland waters. The approach and results of this study provides the potential of effective methods for remote monitoring and management water quality in turbid inland waters using hyperspectral remote sensing. |