| Title |
Evaluation of Alternative Acute Toxicity Test Method with Daphnia magna |
| Authors |
김종민 ( Jongmin Kim ) ; 신기식 ( Kisik Shin ) ; 이정서 ( Jungseo Lee ) ; 이택준 ( Taekjune Lee ) |
| DOI |
https://doi.org/10.15681/KSWE.2017.33.2.181 |
| Keywords |
Alternative test method; D. magna; Toxkits; Wastewater |
| Abstract |
This paper aimed to evaluate the results of an altemative acute toxicity test on 83 wastewater samples. Each sample was tested by a traditional method (laboratory-cultured D. magna as test organisms) and an alternative method (Toxkit, purchased from a specialized company, MicroBioTests Inc., Mariakerke [Gent], Belgium). The relationship between the laboratory-cultured toxicity and Toxkit`s toxicity indicated a strong relationship (r2 =.84, P < .01, N = 83). The number of samples which displayed a small difference of less than .5 between the two test methods was 52 and accounted for 63% of the collected samples. In addition, these 52 samples exhibited a low average toxicity of TU .5 (laboratory culture method) and TU .45 (Toxkit`s method), whereas samples with big differences between the two methods` results displayed a tendency of higher toxicities. These results indicate that the alternative toxicity test method can be applied if samples have a toxicity of less than TU 2. Also, Toxkit`s test results displayed EC50 values between 0.93 and 1.68 mg/L which can be considered to conform with quality control standard. |