| Title |
Detection of Geosmin Production Capability Using geoA Gene in Filamentous Cyanobacteria (Nostocales, Oscillatoriales) Strains |
| Authors |
류희성 ( Hui-seong Ryu ) ; 신라영 ( Ra-young Shin ) ; 서경애 ( Kyung-ae Seo ) ; 이정호 ( Jung-ho Lee ) ; 김경현 ( Kyunghyun Kim ) |
| DOI |
https://doi.org/10.15681/KSWE.2018.34.6.661 |
| Keywords |
Dolichospermum; Nakdong River; Odorous metabolites; Off-flavor; Pseudanabaena |
| Abstract |
Geosmin is volatile metabolites produced by a range of filamentous cyanobacteria which causes taste and odor problems in drinking water. Molecular ecological methods which target biosynthetic genes (geoA) are widely adopted to detect geosmin-producing cyanobacteria. The aim of this study was to investigate the potential production capability of 8 strains isolated from the Nakdong River. Ultimately, a suggestion for a genetical monitoring tool for the identification of geosmin producers in domestic waters was to be made. Geosmin was detected using solid phase microextraction gas chromatography mass spectrometry (SPME GC-MS) in two strains of Dolichospermum plactonicum (DGUC006, DGUC012) that were cultured for 28 day. The highest concentrations during the experiment period was 17,535 ngL-1 and 14,311 ngL-1 respectively. Additionally, geoA genes were amplified using two primers (geo78F/971R and geo78F/982R) from strains shown to produce geosmin, while amplification products were not detected in any of non-producing strains. PCR product (766 bp) was slightly shorter than the expected size for geosmin producers. According to the BLAST analysis, amplified genes were at nucleotide level with Anabaena ucrainica (HQ404996, HQ404997), Dolichospermum planctonicum (KM13400) and Dolichospermum ucrainicum (MF996872) between 99 ~ 100 %. Both strains were thus confirmed as potential geosmin-producing species. We concluded that the molecular method of analysis was a useful tool for monitoring potential cyanobacterial producers of geosmin. |