| Title |
Alternative and Rapid Detection Methods for Wastewater Surveillance of SARS-CoV-2 |
| Authors |
제스민아터 ( Jesmin Akter ) ; 이복진 ( Bokjin Lee ) ; 이재엽 ( Jai-yeop Lee ) ; 안창혁 ( Chang Hyuk Ahn ) ; ( Nishimura Fumitake ) ; 김일호 ( Ilho Kim ) |
| DOI |
https://doi.org/10.15681/KSWE.2024.40.1.19 |
| Keywords |
Rapid detection; RT-LAMP; SARS-CoV-2; Wastewater surveillance |
| Abstract |
The global pandemic, coronavirus disease caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to the implementation of wastewater surveillance as a means to monitor the spread of SARS-CoV-2 prevalence in the community. The challenging aspect of establishing wastewater surveillance requires a well-equipped laboratory for wastewater sample analysis. According to previous studies, RT-PCR-based molecular tests are the most widely used and popular detection method worldwide. However, this approach for the detection or quantification of SARS-CoV-2 from wastewater demands a specialized laboratory, skilled personnel, expensive instruments, and a workflow that typically takes 6 to 8 hours to provide results for a few samples. Rapid and reliable alternative detection methods are needed to enable less-well-qualified practitioners to set up and provide sensitive detection of SARS-CoV-2 within wastewater at regional laboratories. In some cases, the structural and molecular characteristics of SARS-CoV-2 are unknown, and various strategies for the correct diagnosis of COVID-19 have been proposed by research laboratories. The ongoing research and development of alternative and rapid technologies, namely RT-LAMP, ELISA, Biosensors, and GeneXpert, offer a wide range of potential options not only for SARS-CoV-2 detection but also for other viruses. This study aims to discuss the effective regional rapid detection and quantification methods in community wastewater. |