Title |
Bacterial Community Structure in Iron Particle Integrated Anammox Process |
Authors |
이승원 ( Seungwon Lee ) , 박지혜 ( Jihye Park ) , 조민기 ( Minkee Cho ) , 송민수 ( Minsu Song ) , 전준범 ( Junbeom Jeon ) , 배효관 ( Hyokwan Bae ) |
DOI |
https://doi.org/10.15681/KSWE.2024.40.6.316 |
Keywords |
Anaerobic ammonium oxidation; Bacterial community structure; Iron particle integrated anammox granule; Nitrogen removal |
Abstract |
The anaerobic ammonium oxidation (anammox) process has been demonstrated to increase nitrogen removal efficiency and settleability by incorporating iron particles. This study investigated the changes in microbial community structure of the anammox process due to the incorporation of iron particles. Anammox granules (AGs) and iron particle-integrated anammox granules (IP-IAGs) were developed using synthetic wastewater. Then, the real wastewater was applied to partial nitritation and IP-IAGs. The 16S rRNA gene amplicon sequencing revealed that dominant community members were different between each reactor. The dominant anammox genera in IP-IAGs were Ca. Brocadia and Ca. Jettenia. Planctomycetota, Pseudomonadota, Chloroflexi, and Bacteroidota phyla were commonly dominant in AGs and IP-IAGs. However, their relative abundances were changed before and after the iron particles were integrated. In addition, the alpha diversity and evenness of IP-IAGs were higher than AGs, confirming that the bacteria community was more diverse. The dominant anammox bacteria in previous studies were surveyed to explain the characteristics of the anammox community of this study. In addition, this study provides the sequences of suitable primers for quantifying anammox bacteria for the further investigation on the anammox community in iron particle-applied conditions. |