| Keywords |
LDH isozymes ; Diabetes ; Treadmill exercise ; Cytosolic LDH |
| Abstract |
The purpose of this study was to investigate the expression of treadmill exercise on lactate dehydrogenase (LDH) isozymes in the diabetic rats heart. For this study, twenty male F344 rats were assigned to control group (n = 10), control exercise group (n = 10), diabetes group (n = 10) and diabetic exercise group (n = 10). 10 rats were trained according to exercise program for 6 weeks and 10 rats were untrained for control group. The change of cytosolic LDH isozymes activity of cardiac tissue were measured after 6 weeks training in rats. Polyacrylamide gel electrophoresis was performed after exercise training. Relative activity (%) of cardiac cytosolic LDH isozymes for control and exercise group on the gel electrophoresis showed the following order of prevalence : AB3>A2B2> B4>A3B >A4, for diabetic group and diabetic exercise group : AB3> B4>A2B2>A3B >A4. These results suggest that exercise training effect on not only normal cardiac tissue cytosolic LDH isozymes activity but also diabetic cardiac tissue LDH isozymes activity and LDH A4, A3B, A2B2, AB3 and B4 isozymes were found entirely in cytosol, but there was no LDH A4 isozyme in the diabetes rats. |