| Keywords |
Food allergy ; OVA ; RANTES ; RT-PCR ; Immunohistochemistry |
| Abstract |
To clarify the role of chemokine on eosinophil infiltration to gut of ovalbumin (OVA) sensitized rat when challenged with OVA (food allergy model) we made an experiment. For immunohistochemistry, 5 μm cryostatsections were made from the snap-frozen tissue (small intestine) and stained with chromotrope 2R and hematoxylin for eosinophil stain. The number of eosinophils was represented as the average in ten fields at ×400 magnification. In addition, reverse transcription polymerase chain reaction (RT-PCR) and southern blotting were performed to evaluate expression of regulated on activation, normal T cell expressed, and secreted (RANTES) in gut. A marked edema of villi and eosinophil infiltration to the lamina propria was only seen and peaked at 6 h in OVA sensitized mice after the oral challenge with OVA. Furthermore, analysis of RT-PCR and southern blotting demonstrated that RANTES mRNA expression was induced an antigen-specific manner and the expression peaked at 3 h and 6 h and declined thereafter, which is well matched with time course of eosinophil infiltration. Taken together, as in bronchial mucosa and skin, RANTES might play an important role on eosinophil recruitment to the gut site of food allergy in the rats. |