| Keywords |
anaerobic threshold (AT) exercise ; nitric oxide (NO) ; interferon (IFN)-gamma ; interleukin (IL)-1 beta. |
| Abstract |
The influence of exercise at anaerobic threshold (AT) intensity on inflammation response has not been intensively studied. This study attempts to evaluate the effects of exercise at AT intensity on production of nitric oxide (NO), interleukin (IL)-1 beta and interferon (IFN)-gamma in young males. Ten healthy untrained males (age 24.3±2.5 years; BMI 21.3±1.4 kg/m2; VO2max 48.9±7.7 ml/kg/min) completed two trials in a randomized order. In the control trial (CON), the subjects remained in a sitting posture for two hours in thermoneutral conditions. In the exercise at AT intensity trial (EXE), they underwent exercise at AT for approximately 30 min to expend 300 kcal on the cycle ergometer, followed by 90min recovery in thermoneutral conditions. Blood samples were taken before exercise, immediately after exercise and after 90min recovery. The concentrations of NO, IL-1 beta and IFN-gamma in the serum and expression of IL-1 beta mRNA from peripheral blood mononuclear cell (PBMC) were measured. Although serum NO level in CON after 90min recovery was significantly lower than those of immediately after exercise (p<.05), there was no significant difference in EXE at any time period. The change rate (%) of serum NO before AT exercise, however, was shown a tendency of the increase at immediately after exercise and then significantly decreased after 90min recovery (p<.05). There were no significant differences in serum IFN-gamma levels at any trials and time periods (p>.05). On the other hand, although the serum IL-1 beta level was not significant difference, expression of IL-1 beta mRNA of EXE was significant lower than those of CON after 90min recovery (p<.05). Although the change rate (%) of IL-1 beta mRNA in CON was shown a increase after 90min recovery (p<.05), those of EXE were not changed. These results suggested that although a bout of exercise at AT intensity dose not affect on the change of IFN-gamma, it inhibits expression of IL-1 beta mRNA and production of NO. It might be the positive effect on inflammation responses. |