| Keywords |
Diabetes ; Skeletal muscle ; Treadmill running ; p-AMPK ; p-ACC ; Malonyl-CoA |
| Abstract |
This study was to investigated the effects of treadmill exercise on p-AMPK, p-ACC, and Malonyl-CoA expression of skeletal muscle and cardiac muscle in a streptozotocin (STZ)-induced diabetic rats. At 7 weeks of age, S.D rats (n=36) were induced diabetes by administering STZ. A STZ-induced rats were randomly assigned to a control (DM+C, n=12), moderate-intensity exercise group (DM+LE, n=12) and high-intensity exercise group (DM+HE, n=12). The rats were trained progressively on a treadmill, five days per weeks for eight weeks following treadmill running programs: moderate-intensity (15m/min for 60mins) and high-intensity (25m/min for 60mins). After 48-h of the last bout of exercise, blood and tissues of skeletal muscle and heart were harvested. Treadmill running for 8 weeks significantly reduced fasting glucose levels and HOMA-IR in diabetes rat models, regardless of exercise intensity. Both exercise groups significantly increased p-AMPK and p-ACC protein levels. Malonyl-CoA levels were significantly reduced in both exercise groups. The current findings suggest that treadmill running on a STZ-induced diabetes rats can provide an effective means to combat an insulin resistance and improve fatty-acid oxidation (i.e., p-AMPK, p- ACC and malonyl-CoA) in skeletal muscles and heart. |