| Title |
Development of High-Intergrated DNA Array on a Microchip by Fluidic Self-assembly of Particles |
| Authors |
김도균(Kim, Do-Gyun) ; 최용성(Choe, Yong-Seong) ; 권영수(Gwon, Yeong-Su) |
| Keywords |
Random fluidic self-assembly method (RFSA) ; hydrophobic interaction ; Particles ; Pattern-chip ; DNA chip microarrays |
| Abstract |
The DNA chips are devices associating the specific recognition properties of two DNA single strands through hybridization process with the performances of the microtechnology. In the literature, the "Gene chip" or "DNA chip" terminology is employed in a wide way and includes macroarrays and microarrays. Standard definitions are not yet clearly exposed. Generally, the difference between macro and microarray concerns the number of active areas and their size, Macroarrays correspond to devices containing some tens spots of 500μm or larger in diameter. microarrays concern devices containing thousnads spots of size less than 500μm. The key technical parameters for evaluating microarray-manufacturing technologies include microarray density and design, biochemical composition and versatility, repreducibility, throughput, quality, cost and ease of prototyping. Here we report, a new method in which minute particles are arranged in a random fashion on a chip pattern using random fluidic self-assembly (RFSA) method by hydrophobic interaction. We intend to improve the stability of the particles at the time of arrangement by establishing a wall on the chip pattern, besides distinction of an individual particle is enabled by giving a tag structure. This study demonstrates the fabrication of a chip pattern, immobilization of DNA to the particles and arrangement of the minute particle groups on the chip pattern by hydrophobic interaction.ophobic interaction. |