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Title |
Isolation of Microorganisms for Optimization of Autonomous Crack Healing and Verification of Crack Healing
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Authors |
이병재(Byung-Jae Lee) ; 유연준(Yeon-Jun Yu) ; 이효섭(Hyo-Sub Lee) ; 양주경(Joo-Kyoung Yang) ; 이윤(Yun Lee) |
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DOI |
https://doi.org/10.11112/jksmi.2023.27.1.103 |
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Keywords |
자발적 균열치유 콘크리트; 생체광물 형성 작용; 탄산칼슘; 균열치유작용; 미생물; 내생포자 Autonomous healing concrete; Biomineralization; Calcium carbonate; Crack healing; Microorganism; Endospore |
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Abstract |
In this study, basic research was conducted to secure microbial resources applicable to autonomous crack healing concrete. To this end, inthis experiment, biomineral-forming microorganisms were separated from natural sources, and the ability of survival in cement and calcium carbonateprecipitation were compared to secure suitable microbial resources. Bacillus-type bacteria forming endospores were isolated from the sample, and theamount of calcium carbonate produced by the six microorganisms identified by 16S rRNA sequencing was compared. Two types of microorganisms,Bacillus velezensis and Bacillus subtilis, with the highest calcium carbonate precipitation were selected, and the survival of the microorganisms wasconfirmed through phase contrast microscopy after being cured after being added to the mortar. In addition, it was confirmed that the autonomouscrack healing capability by the crack healing material produced by microorganisms was confirmed by artificially generating cracks in the mortar.
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